کنگره کشوری هماتولوژی و مراقبتهای پرستاری از 19 تا 21 آبان ماه در شهر یزد برگزار شد. موضوع سخنرانی اینجانب بررسی اثرات اکسیداتیو آهن بر ساختار هموگلوبین انسان بود.
همکاران طرح آقایان حسن ضیافتی کافی و میلاد محمدی و سرکار خانمها سهیلا رستمی، مریم السادات الحسینی، محیا هاشمی و محدثه سادات عظیمی بودند. عنوان و خلاصه سخنرانی بشرح زیر است:
Spectrophotometric study on the iron-mediated oxidation and structural changes in human hemoglobin molecules
Background: Our previous studies demonstrated the presence of higher level of free radical reaction products in the erythrocytes of menadione and phylloquinone treated subjects. The present study establishes that iron, to be redox cycling active, could play a central role in oxidation of iron-treated erythrocytes
Methods: The blood samples were collected from healthy normal donors and were collected into tubes containing heparin. Human erythrocytes were incubated aerobically with the iron containing metal catalyzed oxidation(MCO) system for 4h. Oxidation of hemoglobin(Hb) gives O2- and methemoglobin(met-Hb). If the globin structure is destabilized, met-Hb converts to hemichrome, the main constituent of Heniz bodies. Structural changes in Hb were followed by spectrophotometric analysis. To calculate the micromolar concentrations of oxy-Hb, met-Hb and hemichrome, the following equations were considered
Oxy-Hb=119A577-39A630 -89A560
Met-Hb=28A577+307A630 -55A560
Hemichrome=-133A577-114A630+233A560
Where A577, A630 and A560 are the absorbance at 577, 630 and 560 nm respectively
Results: According to our findings, oxy-Hb were 68% lower (12.56 µM vs. 18.59µM) in iron-treated erythrocytes compared with control groups. Also a significant increase was seen in the concentration of hemichrome (6.64 µM vs. 0.28 µM). The absorbance ratio(A577/A542) in iron-treated erythrocytes were 0.93 compared with normal subjects (0.97). This decrease indicates the conversion of oxy-Hb to met-Hb
Conclusion: These findings indicate Hb oxidation instead of its oxygenation leading to anemia and hypoxia and suggest that strategies to decrease non-heme iron may significantly reduce oxidative damages to erythrocytes. The relevance of our findings may be directly applicable to oxidative states during hemolytic disease, ischemia reperfusion, and administration of iron
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